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A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
Published on: August 11, 2018
Differential T helper cell response in tuberculous pleuritis.
C Prabha1, K V Jalapathy, R P Matsa
1Department of Immunology, Tuberculosis Research Centre ICMR, Chennai, Tamilnadu, India. sulochanad@icmr.org.in
Indian Journal of Medical Microbiology
|March 23, 2007
Summary
Tuberculous pleuritis exhibits distinct immune responses. In vivo, T-helper 1 (Th1) cells dominate, while in vitro, both Th1 and T-helper 2 (Th2) responses are observed, indicating a mixed cellular immunity.
Area of Science:
- Immunology
- Infectious Diseases
Background:
- Tuberculous pleuritis involves complex immune system responses.
- Understanding systemic versus localized immunity is crucial for effective treatment.
Purpose of the Study:
- To investigate in vivo and in vitro immune responses in tuberculous pleuritis.
- To determine differences between systemic and localized immune responses.
Main Methods:
- Compared in vivo levels of IFN-gamma and IL-4 in plasma and pleural fluid of tuberculous and nontuberculous patients.
- Studied in vitro cytokine response to mycobacterial antigens using ELISA and intracellular cytokine staining on CD4+ T cells.
Main Results:
- Ex vivo analysis revealed increased IFN-gamma and IFN-gamma+ T cells in pleural fluid.
- In vitro studies showed increased IFN-gamma and IL-4 levels upon antigen stimulation, with a Th2-dominant response.
- Antigen-specific increases in both IFN-gamma+ and IL-4+ T cells were observed in vitro.
Conclusions:
- Tuberculous pleuritis demonstrates a differential T-helper response.
- Predominant Th1 response occurs in vivo, while a mixed Th1/Th2 response is seen in vitro.
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