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Updated: Jul 16, 2026

Glycopeptide Capture for Cell Surface Proteomics
Published on: May 9, 2014
Chip-based CE coupled to a quadrupole TOF mass spectrometer for the analysis of a glycopeptide
Yufang Zheng1, Haifang Li, Zhihong Guo
1Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P. R. China.
Abstract:
A novel approach using sheath flow chip-based CE coupled to a quadrupole TOF mass spectrometer was developed and applied to the analysis of an O-glycopeptide. The method provided good separation and sensitive analysis of the glycopeptide and its beta-elimination product. The beta-elimination reaction of the glycopeptide with ammonia or dimethylamine was adapted for changing the glycopeptide into deglycosylated form and for specifying the site of glycosylation. MS/MS analysis of the native peptide, glycopeptide and beta-elimination product enabled the identification of the glycosylation site through the comparison of the fragmentations and peptide sequence analysis.
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