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Co-localization of Cell Lineage Markers and the Tomato Signal

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DMP1-targeted Cre expression in odontoblasts and osteocytes.

Y Lu¹, Y Xie, S Zhang

¹Department of Oral Biology, School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA.

Journal of Dental Research

|March 27, 2007

Summary

Odontoblasts and osteocytes share similar dendritic structures within mineralized tissues. A new Dmp1-Cre mouse model specifically targets these cells, enabling focused gene function studies in both dentin and bone.

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Area of Science:

Cell Biology
Biomineralization
Genetics

Background:

Odontoblasts in dentin and osteocytes in bone possess dendritic processes.
Investigating common features of these cellular processes is crucial for understanding mineralized tissue biology.
Dentin matrix protein 1 (DMP1) is highly expressed in both cell types.

Purpose of the Study:

To compare the cellular morphology of odontoblasts and osteocytes.
To develop and validate a transgenic mouse model for targeted gene manipulation in odontoblasts and osteocytes.

Main Methods:

Scanning electron microscopy was used to visualize cellular morphology.
A transgenic approach utilizing the Dmp1 promoter and intron was employed.
Dmp1-Cre mice were crossed with ROSA26 reporter mice to assess Cre activity specificity and efficiency.

Main Results:

Both odontoblasts and osteocytes exhibit an identical dendritic canalicular system.
Extensive cellular processes form a complex network within the mineralized matrix of dentin and bone.
The Dmp1 promoter-intron construct specifically targeted Cre cDNA expression/deletion in odontoblasts and osteocytes.
Crossing Dmp1-Cre with ROSA26 reporter mice confirmed specific targeting of these cell types.

Conclusions:

Odontoblasts and osteocytes share a common dendritic canalicular system.
A novel Dmp1-Cre transgenic mouse model allows for specific gene function studies in both odontoblasts and osteocytes.
This model provides a valuable tool for investigating the roles of specific genes in dentin and bone biology.