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Published on: February 11, 2021
[Effect of interleukin-1alpha on the phagocytic function in cultured human trabecular meshwork cells]
Xin-yu Li1, Chao-qiong Wang, Nan Xiang
1Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. lixy07@126.com
Objective:
To investigate the effect of interleukin-1alpha (IL-1alpha) on the phagocytic function in cultured human trabecular meshwork (HTM) cells.
Methods:
HTM Cells were cultured and subcultured in vitro. The third to fifth passage of HTM cells were treated with different concentrations of IL-1alpha or vehicle in DMEM/F12 medium. 1000 of 0.85 microm microsphere/per cell were added to each well. The morphological changes of HTM cells were observed by an inverted phase-contrast microscope and electron microscope. The cellular phagocytic function of HTM cells was evaluated by counting the numbers of microsphere in 20 HTM cells using a light microscope.
Results:
The break of HTM cell membrane was found under the electron microscope. The numbers of microsphere in HTM cells was significantly (P = 0.005) reduced in a dose- and incubation time-dependent manner (F = 3.603, P = 0.046, F = 15.846, P = 0.005, respectively).
Conclusions:
IL-1alpha affects the phagocytic function of HTM cells that is correlated to the concentration and the incubation time.
