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Published on: November 10, 2023
[Experimental study of the permeability of Fel Ursi's in eye]
Hao Cui1, Jing-jing Liu, Shao-ying Fu
1Eye Hospital, First Clinical College of Harbin Medical University, Harbin 150001, China.
Objective:
To determine whether Tauroursodeoxycholic acid (TUDCA) can penetrate the blood-ocular barrier after orally administration of Fel Ursi.
Methods:
56 rabbits were divided into two groups, 48 rabbits were used in experimental group, and other 8 rabbits were served as control. 100 mg/ml Fel Ursi were a fused into rabbits stomach. 2 ml blood from vein of auris-edge, aqueous humor from left eye and vitreous sample from right eye were obtained at 0.5, 1.0, 2.0, 4.0, 6.0 and 8.0 h after Fel Ursi administration. Concentration of TUDCA from all of samples was determined by HPLC.
Results:
TUDCA Concentrations were (999.1 +/- 17.2) - (1300.6 +/- 78.2) microg/ml, (12.7 +/- 1.4) - (47.8 +/- 4.7) microg/ml, and (10.8 +/- 2.9) - (57.9 +/- 7.9) microg/ml in blood, aqueous humor and vitreous respectively. There was no significant differences in the concentration of TUDCA in samples of aqueous humor and vitreous (P > 0.05). However the concentration of TUDCA in rabbit blood was much higher compared with that in aqueous humor and vitreous (P < 0.01).
Conclusion:
Fel Ursi can reach intraocular tissue through penetrating blood-aqueous barrier and blood-vitreous barrier after orally application.
