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Changes occurring on the cell surface during KSHV reactivation.

Audy G Whitman1, Ossie F Dyson, Phelps J Lambert

  • 1Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834, USA.

Journal of Electron Microscopy
|March 30, 2007
PubMed
Summary
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Kaposi

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Kaposi's sarcoma-associated herpes virus (KSHV) infection primarily remains latent.
  • Lytic reactivation of KSHV is infrequent, occurring in 1-2% of infected cells.
  • Previous studies indicate a link between KSHV lytic reactivation and cell cycle progression.

Purpose of the Study:

  • To investigate the surface changes of cells during KSHV reactivation using scanning electron microscopy (SEM).
  • To explore the relationship between cell surface topology, cell cycle, and KSHV reactivation.

Main Methods:

  • Scanning electron microscopy (SEM) was employed to examine cellular surface morphology during KSHV reactivation.
  • Transmission electron microscopy (TEM) provided further evidence of viral egress mechanisms.

Related Experiment Videos

Main Results:

  • KSHV reactivation was observed to occur preferentially in cells exhibiting a smoother surface topology, characteristic of S-phase cells.
  • KSHV particles were visualized budding from infected cells during the late stages of reactivation.
  • SEM and TEM provided evidence linking cell surface characteristics to KSHV reactivation.

Conclusions:

  • This study establishes a direct correlation between cell surface topology, cell cycle progression (specifically S phase), and KSHV lytic reactivation.
  • Viral egress via budding was observed during KSHV reactivation.