Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Burn depth estimation using indocyanine green fluorescence.

H A Green1, D Bua, R R Anderson

  • 1Harvard Medical School, Department of Dermatology, Massachusetts General Hospital, Boston 02114.

Archives of Dermatology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety and efficacy of endoscopic spray cryotherapy for esophageal cancer.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2017
Same author

Laser-induced thermal coagulation enhances skin uptake of topically applied compounds.

Lasers in surgery and medicine·2017
Same author

Fractional laser-assisted drug delivery: Laser channel depth influences biodistribution and skin deposition of methotrexate.

Lasers in surgery and medicine·2016
Same author

Ablative fractional laser alters biodistribution of ingenol mebutate in the skin.

Archives of dermatological research·2015
Same author

Novel methods for generating fractional epidermal micrografts.

The British journal of dermatology·2014
Same author

Fractional laser-mediated photodynamic therapy of high-risk basal cell carcinomas--a randomized clinical trial.

The British journal of dermatology·2014
Same journal

Scoring With SCORTEN-Reply.

Archives of dermatology·2017
Same journal

Comparison of Early and Late Latent Syphilis- Colorado, 1991.

Archives of dermatology·2016
Same journal

Case of Rhinoscleroma.

Archives of dermatology·2016
Same journal

Cutaneous Fungal Infections.

Archives of dermatology·2016
Same journal

An Ulcerated Plaque in a Gay Man.

Archives of dermatology·2016
Same journal

Solitary Violaceous Nodule on the Face.

Archives of dermatology·2016
See all related articles

A new infrared fluorescence technique using indocyanine green can accurately distinguish between full-thickness and partial-thickness burns. This method aids in early surgical planning and treatment for burn wound depth assessment.

Area of Science:

  • Biomedical Optics
  • Dermatology
  • Medical Imaging

Background:

  • Accurate burn depth assessment is critical for effective wound management and surgical planning.
  • Current methods for differentiating burn depths often lack precision, leading to suboptimal treatment outcomes.
  • Early identification of full-thickness burns is essential for timely excision and grafting.

Purpose of the Study:

  • To introduce and validate a novel technique for determining burn wound depth.
  • To assess the efficacy of infrared fluorescence imaging with indocyanine green in distinguishing burn depths.
  • To provide an early diagnostic tool for surgical decision-making in burn care.

Main Methods:

  • Utilized indocyanine green (ICG) administered intravenously.

Related Experiment Videos

  • Applied dual-wavelength excitation (infrared at 780 nm and UV at 369 nm) to induce ICG fluorescence.
  • Measured infrared fluorescence emission ratios in hairless rat skin models with varying burn depths (partial-thickness and full-thickness) at multiple time points post-burn (immediately, days 1-3, and day 7).

    • Main Results:

    • The ratio of infrared-induced to UV-induced fluorescence intensity effectively differentiated between full-thickness and partial-thickness burns.
    • This distinction was achievable immediately after burn injury and persisted through day 7.
    • The technique demonstrated high sensitivity in identifying burn depth.

    Conclusions:

    • Dual-wavelength excitation of indocyanine green fluorescence provides a reliable method for early burn depth assessment.
    • This technique can significantly improve the accuracy of differentiating burn wound types.
    • Enables better prognosis, surgical planning, and timely intervention, including primary excision and grafting.