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Updated: Jul 16, 2026
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Experimental and Imaging Techniques for Examining Fibrin Clot Structures in Normal and Diseased States
Published on: April 1, 2015
Fribrinolysis kinetics and its application
Wenning Wei1, Rui Yang, Tao Guo
1Institution of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. wenningwei@yahoo.com.cn
Summary
A novel spectrophotometer method accurately measures human fibrinolysis kinetics. This rapid technique differentiates normal subjects from leukemia patients, aiding clinical decisions.
Area of Science:
- Biochemistry
- Hematology
- Analytical Chemistry
Background:
- Fibrinolysis is a critical physiological process for blood clot removal.
- Accurate kinetic measurement of fibrinolysis is essential for diagnosing and managing hemostatic disorders.
- Existing methods may lack convenience, speed, or comprehensive data output.
Purpose of the Study:
- To establish a new, convenient, and rapid method for the kinetic measurement of human fibrinolysis.
- To determine optimal parameters for the assay, including urokinase concentration.
- To evaluate the method's utility in distinguishing between normal individuals and patients with specific hematological conditions.
Main Methods:
- Utilized a UV2000 spectrophotometer connected to a computer for automatic scanning and recording of absorbance changes during coagulation and lysis.
- Established key parameters for human fibrinolysis kinetics.
- Tested urokinase at various concentrations to find the optimal concentration (20 U/mL).
Main Results:
- The optimized method demonstrated a coefficient of variation of (5.24+/-1.51)%.
- Significant differences in fibrinolysis kinetics and plasma plasminogen concentration were observed between 22 normal subjects and 27 acute myeloblastic leukemia patients (P<0.05 and P<0.01, respectively).
- The method simultaneously allowed for the measurement of plasma fibrinogen concentration.
Conclusions:
- The developed method is stable, providing dynamic, direct experimental data and multiple parameters for clinical use.
- This assay is valuable for evaluating anti- and pro-fibrinolytic capacity in patient plasmas.
- The method supports therapy monitoring, drug selection, and dosage adjustments in clinical practice.
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