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Noninvasive identification of different intraocular lenses using Raman spectroscopy in porcine eyes
Jong-uk Hwang1, Heejeong Choi, Dae Hong Jeong
1Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Songpa-Gu, Seoul, Korea.
Purpose:
To develop a noninvasive technique for identifying the specific type of intraocular lens (IOL) implanted in porcine eyes.
Setting:
Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, and Department of Chemistry Education, Seoul National University, Seoul, Korea.
Methods:
A confocal Raman spectroscopy system was used to identify various acrylic (AcrySof SA60AT [Alcon], Sensar AR40e [Advanced Medical Optics], I-Flex [I-Medical Intraocular Lenses]) and silicone (ClariFlex, [Advanced Medical Optics]) IOLs implanted in enucleated pig eyes. Confocal Raman spectra of IOLs were obtained by photoexcitation using the 514.5 nm line from an argon-ion laser, with a power of 1 mW at the sample and an acquisition time of 10 to 100 seconds.
Results:
All IOLs, including those composed of acrylic optic material from different manufacturers, exhibited distinct spectral peaks before and after implantation in pig eyes.
Conclusion:
Confocal Raman spectroscopy may represent a novel method for obtaining specific information about implanted IOLs through direct and noninvasive confocal observation of spectral characteristics.
