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Esterase 2 from Alicyclobacillus acidocaldarius as a reporter and affinity tag for expression and single step

Yiwei Huang1, Martin Humenik, Mathias Sprinzl

  • 1Laboratorium für Biochemie, Universität Bayreuth, 95440 Bayreuth, Germany.

Protein Expression and Purification
|April 3, 2007
PubMed

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...