[Production of essential oils by cell-suspensions of Matricaria chamomilla in a two phase system.]

Cell suspensions of Matricaria chamomilla were cultured in a two phase system consisting of an aqueous nutrient medium and a nontoxic lipophilic phase (triglyceride). Especially during the first week of culture period a great number of lipophilic substances were accumulated in the triglyceride phase as was demonstrated by TLC and GC. One of these substances could be identified as alpha-Bisabolol using GC-MS-coupling. Cell growth was unimpaired by the addition of the lipophilic phase to the nutrient medium.