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Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
Published on: May 18, 2015
Comparison of polyester scaffolds for bioengineered intestinal mucosa
David C Chen1, Jeffrey R Avansino, Vatche G Agopian
1Department of Surgery, VA Greater Los Angeles Health Care System, University of California at Los Angeles, Los Angeles, Calif 90024, USA.
Cells, Tissues, Organs
|April 6, 2007
Summary
Biodegradable scaffolds successfully supported intestinal stem cell engraftment and mucosal layer formation in rats. Scaffold material and fabrication influenced engraftment success, highlighting potential for tissue-engineered intestine development.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Biodegradable polyester scaffolds facilitate 3D cell growth for neointestinal tissue engineering.
- Scaffolds maintain nutrient flow crucial for cell viability and tissue development.
Purpose of the Study:
- To evaluate various biopolymer constructs for neointestinal tissue engineering.
- To determine mucosal engraftment rates and morphology on different scaffolds.
Main Methods:
- Eight biodegradable polymer tubes (polyglycolic acid, polylactic acid, or combination) were fabricated.
- Neonatal rat intestinal stem cell clusters seeded constructs were implanted into rat omentum for 4 weeks.
- Grafts were analyzed for mucosal growth, morphology, scarring, and residual polymer.
Main Results:
- Mucosal engraftment occurred in 7 of 8 constructs.
- Optimal engraftment (36% surface area) and morphology with villous/crypt development were observed on nonwoven polyglycolic acid mesh coated with poly-L-lactic acid.
- Electrospun microfiber polyglycolic acid showed poor engraftment and minimal tissue formation.
Conclusions:
- Intestinal organoids can be successfully engrafted onto biodegradable polyester scaffolds, restoring an intestinal mucosal layer.
- Scaffold composition, fabrication, and material properties significantly impact engraftment success.
- Further material optimization is essential for advancing tissue-engineered intestine development.
