Related Experiment Video
Updated: Aug 19, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
Published on: November 27, 2013
cDNA sequence of a growth factor-inducible immediate early gene and characterization of its encoded protein
C H Charles1, A S Abler, L F Lau
1Department of Genetics, University of Illinois College of Medicine, Chicago 60612.
Abstract:
We report the cDNA sequence of 3CH134, an immediate early gene whose transcription is rapidly and transiently stimulated by serum growth factors. 3CH134 encodes a 367 amino acid protein that does not share significant sequence similarity with any known protein. 3CH134 is inducible through multiple signal transduction pathways, and in the adult mouse is expressed predominantly in the lung. Using affinity-purified antibodies, we have identified the 3CH134 protein in serum-stimulated Balb/c 3T3 cells and determined that it has a short half-life.
More Related Videos
08:51Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
09:32Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
Related Concept Videos
RNA Polymerase II Accessory Proteins
Cell Specific Gene Expression
Cis-regulatory Sequences
Reporter Genes
Commonly used reporter...
TGF - β Signaling Pathway
Activation and Inactivation of G Proteins