Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A human Id-like helix-loop-helix protein expressed during early development.

J Biggs1, E V Murphy, M A Israel

  • 1Preuss Laboratory for Molecular Neuro-oncology, Department of Neurological Surgery, School of Medicine, University of California, Box 0520, San Francisco 94143.

Proceedings of the National Academy of Sciences of the United States of America
|February 15, 1992
PubMed
Summary

A newly identified human helix-loop-helix (HLH) gene, Id-2, is highly expressed during early development, particularly in the central nervous system. Id-2 likely inhibits tissue-specific gene expression, with a developmental role distinct from the related Id protein.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Afterslip Moment Scaling and Variability From a Global Compilation of Estimates.

Journal of geophysical research. Solid earth·2022
Same author

Orbit period modulation for relative motion using continuous low thrust in the two-body and restricted three-body problems.

Celestial mechanics and dynamical astronomy·2020
Same author

Secretion-mediated STAT3 activation promotes self-renewal of glioma stem-like cells during hypoxia.

Oncogene·2017
Same author

Global link between deformation and volcanic eruption quantified by satellite imagery.

Nature communications·2014
Same author

Id4 suppresses MMP2-mediated invasion of glioblastoma-derived cells by direct inactivation of Twist1 function.

Oncogene·2014
Same author

Geophysics. Monitoring volcanoes.

Science (New York, N.Y.)·2012

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Helix-loop-helix (HLH) proteins regulate tissue differentiation, such as in mammalian muscle and insect nervous systems.
  • Myogenic HLH proteins (e.g., MyoD) and ubiquitous HLH proteins (e.g., E12) form DNA-binding heterodimers to activate muscle-specific genes.
  • The inhibitor of differentiation (Id) protein prevents HLH protein dimerization in proliferating myoblasts.

Purpose of the Study:

  • To report the sequence and structure of a novel human HLH gene related to Id, designated Id-2.
  • To investigate the expression patterns and potential function of the Id-2 gene and protein during human development.

Main Methods:

  • Sequence analysis of the human Id-2 gene.
  • RNA analysis to detect Id-2 mRNA transcripts in various human tissues.

Related Experiment Videos

  • Investigation of Id-2 expression during fetal development and in neuroblastoma cell differentiation.

    • Main Results:

    • The human Id-2 gene encodes a 134-amino acid protein with high homology in the HLH region to myogenic Id.
    • Two prominent Id-2 RNA transcripts (2.5 and 1.3 kb) were detected in normal and neoplastic human tissues.
    • Id-2 is highly expressed in early fetal tissues, including the central nervous system, but not in mature tissues.
    • Id-2 expression is modulated during retinoic acid-induced differentiation of neuroblastoma cells.

    Conclusions:

    • Id-2 functions as an inhibitor of tissue-specific gene expression.
    • The distinct developmental expression pattern of Id-2 suggests a role separate from the Id protein.
    • Id-2 may play a crucial role in early development, particularly in the central nervous system.