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Preparation of Functional Silica Using a Bioinspired Method
Published on: August 1, 2018
Silica-immobilized enzymes for multi-step synthesis in microfluidic devices
Heather R Luckarift1, Bosung S Ku, Jonathan S Dordick
1Air Force Research Laboratory, 139 Barnes Drive, Tyndall AFB, Florida 32403, USA.
Biotechnology and Bioengineering
|April 7, 2007
Summary
This study reports the continuous flow synthesis of 2-aminophenoxazin-3-one (APO) using a microfluidic chemo-enzymatic system. The novel approach integrates metallic zinc and immobilized enzymes for efficient natural product synthesis from nitrobenzene.
Area of Science:
- Chemical Engineering
- Biocatalysis
- Microfluidics
Background:
- 2-aminophenoxazin-3-one (APO) is a complex natural product.
- Traditional synthesis methods can be inefficient and complex.
- Microfluidic systems offer advantages for controlled chemical synthesis.
Purpose of the Study:
- To develop a continuous flow chemo-enzymatic system for APO synthesis.
- To integrate metallic zinc and immobilized enzymes in a microfluidic device.
- To demonstrate the efficient synthesis of APO from nitrobenzene.
Main Methods:
- A microfluidic device was constructed with serially connected chips.
- Chips contained metallic zinc, silica-immobilized hydroxylaminobenzene mutase, and silica-immobilized soybean peroxidase.
- Nitrobenzene was used as the substrate for continuous flow synthesis.
Main Results:
- The chemo-enzymatic system successfully synthesized 2-aminophenoxazin-3-one (APO).
- Silica-immobilization facilitated enzyme stabilization and integration in the microfluidic device.
- The system enabled efficient conversion of nitrobenzene to APO under continuous flow.
Conclusions:
- A novel microfluidic chemo-enzymatic system was established for APO synthesis.
- The system provides a streamlined and efficient method for natural product synthesis.
- This approach demonstrates the potential of integrated microfluidic systems in biocatalysis.
