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Updated: Jul 15, 2026

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
Published on: October 17, 2025
Assay optimization: a statistical design of experiments approach
Maneesha Altekar1, Carol A Homon, Mohammed A Kashem
1Hercules, 1313 North Market Street, Wilmington, DE 19894-0001, USA.
Abstract:
With the transition from manual to robotic HTS in the last several years, assay optimization has become a significant bottleneck. Recent advances in robotic liquid handling have made it feasible to reduce assay optimization timelines with the application of statistically designed experiments. When implemented, they can efficiently optimize assays by rapidly identifying significant factors, complex interactions, and nonlinear responses. This article focuses on the use of statistically designed experiments in assay optimization.
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