Optimization of levofloxacin analysis by RP-HPLC using multivariate calibration technique

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Levofloxacin is a widely used antibiotic.
• Accurate quantification of levofloxacin is crucial for therapeutic drug monitoring and quality control.

Purpose of the Study:

• To develop and validate a rapid and sensitive RP-HPLC method for levofloxacin analysis.
• To establish a robust method for quantifying levofloxacin in bulk materials, pharmaceutical formulations, and human serum.

Main Methods:

• Utilized a Nucleosil C18 column with an isocratic mobile phase of water and acetonitrile.
• Optimized analysis using multivariate calibration and recorded detector response at multiple wavelengths.
• Employed propylparaben as an internal standard for enhanced accuracy.

Main Results:

• Achieved a linear response (r > 0.9999) over a wide concentration range (40-10000 ng/ml).
• Demonstrated excellent recoveries and low intra/inter-day relative standard deviations (<1.2%).
• Validated method parameters including specificity, accuracy, and robustness.

Conclusions:

• The developed RP-HPLC method is rapid, sensitive, and suitable for routine analysis of levofloxacin.
• This method can be reliably applied to determine levofloxacin pharmacokinetic levels in human serum and pharmaceutical products.