Pregabalin and tranexamic Acid evaluation by two simple and sensitive spectrophotometric methods

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Accurate quantification of pharmaceuticals like tranexamic acid and pregabalin is crucial for quality control.
• Existing analytical methods may require complex procedures or specialized equipment.

Purpose of the Study:

• To develop and validate simple, sensitive, and cost-effective colorimetric spectrophotometric methods for quantifying tranexamic acid and pregabalin.
• To investigate the reaction mechanisms and thermodynamic properties of the developed methods.

Main Methods:

• Colorimetric quantification using visible spectrophotometry.
• Reaction of amino group in tranexamic acid and pregabalin with 2,4-dinitrophenol and 2,4,6-trinitrophenol.
• Optimization of reaction conditions, stoichiometric studies, and thermodynamic analysis.

Main Results:

• Yellow colored complexes formed with absorption maxima at 418 nm and 425 nm.
• Linearity over a wide concentration range (0.02-200 µg/mL) with R² > 0.9990.
• Low limits of detection and quantification, excellent recovery, and no excipient interference.

Conclusions:

• The proposed spectrophotometric methods are reliable and suitable for the determination of tranexamic acid and pregabalin in commercial formulations.
• The methods demonstrate high accuracy and precision, comparable to reference techniques.