Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Mutagenesis with Drosophila.

Patrick Emery¹

¹Department of Neurobiology, University of Massachusetts Medical School, Worcester, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 10, 2007

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Membrane proteomics of the <i>Drosophila</i> circadian neural network.

bioRxiv : the preprint server for biology·2026

Same author

Diet-dependent sleep modulation by the <i>Drosophila</i> amino acid transporter ANIDRA.

bioRxiv : the preprint server for biology·2026

Same author

The CK2 and DBT kinases promote temperature compensation of the <i>Drosophila</i> circadian clock via distinct pathways.

bioRxiv : the preprint server for biology·2026

Same author

Core circadian clock genes control molecular and behavioral circatidal rhythms in <i>Parhyale hawaiensis</i>.

bioRxiv : the preprint server for biology·2026

Same author

<i>miR-124</i> acts During <i>Drosophila</i> Development to Determine the Phase of Adult Circadian Behavior.

Journal of biological rhythms·2025

Same author

Biological rhythms: In the sea, two clocks are better than one.

Current biology : CB·2025

Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Forward genetics in Drosophila has identified key circadian rhythm genes by isolating variants that alter the pacemaker

Area of Science:

Chronobiology
Genetics
Animal Model Organisms

Background:

Forward genetics in Drosophila has been crucial for understanding circadian rhythms.
Most Drosophila pacemaker genes were found by studying variants affecting the circadian pacemaker's free-running period.

Purpose of the Study:

To highlight the importance of forward genetics in Drosophila for circadian rhythm research.
To discuss complementary methods for mutagenizing flies to identify pacemaker genes.

Main Methods:

Utilizing forward genetics approaches in Drosophila.
Employing random mutagenesis with alkylating agents to alter the fly genome.
Mobilizing transposable elements to disrupt or modulate gene expression.

Related Experiment Videos

Main Results:

Discovery of numerous Drosophila pacemaker genes through genetic screens.
Identification of gene variants impacting the circadian pacemaker's free-running period.

Conclusions:

Forward genetics in Drosophila is a powerful tool for dissecting complex biological processes like circadian rhythms.
Different mutagenesis strategies offer complementary advantages for gene discovery in Drosophila.