Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
RNA Structure01:23

RNA Structure

79.2K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.2K
RNA Stability01:53

RNA Stability

35.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
RNA Splicing01:32

RNA Splicing

60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
Alternative RNA Splicing02:18

Alternative RNA Splicing

25.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simulated natural daylight and twilight modulate activity and light sampling behaviour in mice.

BMC biology·2026
Same author

Analgesia through FKBP51 inhibition at disease onset confers lasting relief from sensory and emotional chronic pain symptoms.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Optogenetic vision restoration in the face of secondary and tertiary remodeling in the rd1 mouse retina.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Deficient synaptic neurotransmission results in a persistent sleep-like cortical activity across vigilance states in mice.

Current biology : CB·2025
Same author

Hypotheses in light detection by vertebrate ancient opsin in the bird brain.

Journal of neuroendocrinology·2025
Same author

Evaluation of the Digital Ventilated Cage® system for circadian phenotyping.

Scientific reports·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
06:06

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Published on: July 22, 2021

6.9K

RNA extraction from mammalian tissues.

Stuart N Peirson1, Jason N Butler

  • 1Division of Circadian and Visual Neuroscience, University of Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2007
PubMed
Summary
This summary is machine-generated.

This guide details RNA extraction using phenol-chloroform, emphasizing RNase contamination prevention for high-quality nucleic acid purification essential for molecular biology. It covers the entire process from tissue collection to quantification.

More Related Videos

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
10:12

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Published on: September 19, 2020

9.9K
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

52.0K

Related Experiment Videos

Last Updated: Feb 10, 2026

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
06:06

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Published on: July 22, 2021

6.9K
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
10:12

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Published on: September 19, 2020

9.9K
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

52.0K

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Intact RNA purification is crucial for molecular biology techniques like PCR and Northern blotting.
  • RNA extraction methods include phenol-chloroform and solid-phase approaches.
  • RNase contamination is a significant challenge in RNA isolation.

Purpose of the Study:

  • To provide guidelines for reproducible RNA extraction from various tissues.
  • To focus on the phenol-chloroform method for RNA isolation.
  • To address the prevention of RNase contamination during RNA purification.

Main Methods:

  • Phenol-chloroform RNA extraction.
  • Tissue collection and homogenization.
  • RNA quantification.

Main Results:

  • The phenol-chloroform method is versatile and adaptable to different tissue types.
  • Careful procedures and countermeasures can mitigate RNase contamination.
  • High-quality RNA can be reproducibly extracted.

Conclusions:

  • Effective RNA isolation requires meticulous attention to RNase prevention.
  • The phenol-chloroform method, when performed correctly, yields high-quality RNA.
  • This protocol supports downstream molecular biology applications.