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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jul 15, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

Reporter assays.

M Fernanda Ceriani1

  • 1Department Behavioral Genetics, Fundación Instituto Leloir, Buenos Aires, Argentina.

Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2007
PubMed
Summary

This study presents a method for testing gene transcription activators and repressors. The transcriptional assay uses transiently transfected Schneider's cells for functional analysis of molecular clockworks.

Area of Science:

  • Molecular Biology
  • Genetics
  • Chronobiology

Background:

  • Transcriptional feedback loops are fundamental to the molecular mechanisms underlying biological clocks.
  • The cloning of individual clock genes necessitated methods for assessing their regulatory functions.

Purpose of the Study:

  • To describe and provide guidelines for a transcriptional assay.
  • To enable functional testing of potential transcription activators and repressors.

Main Methods:

  • Utilizing transiently transfected Schneider's cells.
  • Performing transcriptional assays to evaluate gene regulation.

Main Results:

  • The described assay allows for direct functional testing of transcription factors.

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Last Updated: Jul 15, 2026

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Published on: August 17, 2014

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  • This method is applicable to the study of molecular clockworks.

    • Conclusions:

    • A straightforward transcriptional assay in Schneider's cells is presented.
    • This assay facilitates the functional characterization of genes involved in transcriptional feedback loops.