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Related Experiment Videos

Cdk1 coordinates cell-surface growth with the cell cycle.

Derek McCusker1, Carilee Denison, Scott Anderson

  • 1Department of Molecular, Cell and Developmental Biology, University of California, 1156 High Street, Santa Cruz, CA 95064, USA.

Nature Cell Biology
|April 10, 2007
PubMed
Summary
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Cyclin-dependent kinase 1 (Cdk1) unexpectedly controls budding yeast bud growth after emergence. G1 cyclin-Cdk1 complexes phosphorylate Cdc24-associated proteins, linking Cdk1 to polarized cell growth.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell cycle progression is regulated by complex mechanisms.
  • Cyclin-dependent kinase 1 (Cdk1) activates Cdc42 GTPase for bud emergence in budding yeast.
  • Cdk1's role in post-emergence bud growth is not well-defined.

Purpose of the Study:

  • To investigate the role of Cdk1 in bud growth after emergence.
  • To identify Cdk1 targets involved in regulating bud growth.
  • To elucidate the mechanism linking Cdk1 to polarized cell growth.

Main Methods:

  • Utilized budding yeast as a model organism.
  • Investigated Cdk1 activity and its effects on bud growth.
  • Performed phosphoproteomic analysis to identify Cdk1 substrates.

Related Experiment Videos

  • Generated and analyzed mutants affecting Cdk1-dependent phosphorylation of Cdc24-associated proteins.

    • Main Results:

    • Demonstrated an unexpected role for Cdk1 in controlling bud growth post-emergence.
    • Identified specific phosphorylation of Cdc24-associated proteins by G1 cyclin-Cdk1 complexes.
    • Showed that impaired Cdk1-dependent phosphorylation of a Cdc24-associated protein leads to bud growth defects.

    Conclusions:

    • Cdk1 activity is crucial for regulating bud growth after bud emergence.
    • G1 cyclin-Cdk1 complexes directly influence the Cdc42 GTPase pathway via phosphorylation of Cdc24-associated proteins.
    • These findings establish a direct link between Cdk1 and the control of polarized cell growth.