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Automation of solid-phase microextraction on a 96-well plate format.
Joseph P Hutchinson1, Lucie Setkova, Janusz Pawliszyn
1Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
Journal of Chromatography. A
|April 10, 2007
Summary
Automated solid-phase microextraction (SPME) using a 96-well plate format was developed for high-throughput analysis. This method efficiently extracts polycyclic aromatic hydrocarbons (PAHs) using polydimethylsiloxane (PDMS) and is compatible with chromatography.
Area of Science:
- Analytical Chemistry
- Environmental Science
Background:
- Solid-phase microextraction (SPME) is a versatile technique for sample preparation.
- Automation of SPME is crucial for increasing sample throughput in analytical laboratories.
Purpose of the Study:
- To assess the feasibility and characterize the automation of SPME in a multi-well plate format.
- To develop a high-throughput SPME method for analyzing polycyclic aromatic hydrocarbons (PAHs).
Main Methods:
- Investigated four polydimethylsiloxane (PDMS) configurations for SPME.
- Developed a 96-SPME device array for parallel microextractions.
- Optimized agitation (orbital shaking) and desorption conditions for liquid-phase SPME.
Main Results:
- A stainless steel pin covered with silicone tubing was identified as a robust SPME configuration.
- Orbital shaking at a stationary position proved optimal for liquid SPME.
- Demonstrated successful extraction and desorption of four PAHs (naphthalene, fluorene, anthracene, fluoranthene).
Conclusions:
- Automated liquid-phase SPME in a 96-well plate format is a viable high-throughput analytical method.
- The developed method is compatible with both gas and liquid chromatography platforms.
- This approach significantly enhances efficiency for analyzing PAHs and similar compounds.
