Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-Functional Silent-Region SERS Nanoplatform for Real-Time Bacterial Tracking and Wound Healing Therapy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Simultaneous Nucleation-Nanowelding Generates Uniform Plasmonic Au-Au Nanojunctions in 3D-Welded Gold Nanostructures.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Robust SERS Platform Enabled by Silver-Nanoparticle-Loaded Hydrogel Millibeads for Trace Detection in High-Salt and Protein-Rich Complex Matrixes.

Nano letters·2026
Same author

Electrophoresis-assisted SERS-LFA strip for simultaneous HIV and HBV detection.

Biosensors & bioelectronics·2026
Same author

Advances in intelligent multi-mode lateral flow assays: from multi-metallic nanomaterials to smart analytical integration.

Chemical Society reviews·2026
Same author

Vertically Stacked Paper-Based Microarray Device for High-Throughput SERS Detection of Two Cancer Biomarkers.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: Jul 15, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Rapid DNA hybridization analysis using a PDMS microfluidic sensor and a molecular beacon.

Sungyong Kim1, Lingxin Chen, Sangyeop Lee

  • 1Department of Applied Chemistry, Hanyang University, South Korea.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|April 11, 2007
PubMed
Summary

This study introduces a novel DNA microfluidic sensor using a molecular beacon for rapid DNA analysis. This method eliminates the need for fluorescent labeling of target DNA, enhancing diagnostic capabilities.

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: Jul 15, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Previous DNA hybridization analysis methods using PDMS microfluidic sensors relied on fluorescence energy transfer (FRET).
  • These FRET-based methods required target DNA to be labeled with fluorescent dyes, limiting their application in real-world samples.

Purpose of the Study:

  • To develop a new DNA microfluidic sensor for rapid DNA analysis.
  • To overcome the limitation of target DNA labeling in previous FRET-based methods.
  • To create a diagnostic tool for efficient DNA hybridization detection.

Main Methods:

  • Development of a novel DNA microfluidic sensor utilizing a molecular beacon.
  • Monitoring changes in fluorescence intensity along the microfluidic channel.
  • Utilizing polydimethylsiloxane (PDMS) for microfluidic channel fabrication.

Main Results:

  • The molecular beacon sensor successfully detected DNA hybridization without labeling the target DNA.
  • Changes in restored fluorescence intensity correlated with successful hybridization.
  • Demonstrated rapid DNA analysis capabilities.

Conclusions:

  • The developed molecular beacon-based microfluidic sensor offers a promising approach for rapid DNA hybridization analysis.
  • This method eliminates the need for target DNA labeling, simplifying sample preparation.
  • The sensor shows potential as a diagnostic tool for various DNA analysis applications.