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Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
Published on: August 26, 2014
Systematic isolation and characterization of cDNAs encoding AAA proteins from human brain.
Xinping Zhao1, P Hedera, J K Fink
1Department of Neurology, University of Michigan Medical Center, Ann Arbor, Michigan, USA.
Bratislavske Lekarske Listy
|April 12, 2007
Summary
Researchers identified 14 novel ATPases Associated with various cellular Activities (AAA) proteins in the human brain. These proteins are potential candidates for causing hereditary spastic paraplegia (HSP) and other neurodegenerative disorders.
Area of Science:
- Molecular Biology
- Genetics
- Neuroscience
Background:
- ATPases Associated with various cellular Activities (AAA) proteins are crucial in cellular functions.
- Mutations in AAA-domain genes are linked to various human diseases, including hereditary spastic paraplegia (HSP).
- HSP is a group of degenerative spinal cord disorders with overlapping clinical presentations.
Purpose of the Study:
- To identify novel AAA-containing proteins as potential candidates for HSP and related neurodegenerative disorders.
- To investigate the role of AAA proteins in neurological diseases.
Main Methods:
- Degenerative PCR was employed, targeting conserved AAA peptide sequences.
- Systematic cloning and characterization of AAA genes expressed in the human brain were performed.
- Analysis of 646 clones to identify known and novel AAA-containing proteins.
Main Results:
- Nineteen known AAA-containing proteins, including spastin and paraplegin (implicated in HSP), were identified.
- Fourteen unique DNA inserts representing novel putative AAA-containing proteins were discovered.
- Four of these novel genes are hypothetical AAA proteins; the others match uncharacterized expressed sequence tags (ESTs).
Conclusions:
- Fourteen novel AAA-containing proteins represent potential candidates for human diseases.
- These novel proteins may play a role in degenerative neurologic disorders.
- Further analysis of these novel AAA proteins is currently underway.
