Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Steric crowding effects on target detection in an affinity biosensor.

Lisa M Bonanno1, Lisa A Delouise

  • 1Department of Biomedical Engineering, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, New York 14642, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|April 12, 2007
PubMed
Summary

Steric crowding impacts biosensor performance by hindering antibody immobilization and target detection. Optimizing the biotin/streptavidin system on porous silicon surfaces is key for enhanced biosensor design.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Examining the immunomodulatory role of nanoparticles on mast cell activation.

Scientific reports·2026
Same author

Design, fabrication, and characterization of a microbubble array microphysiological system.

Lab on a chip·2025
Same author

Salivary gland tissue chip screening identifies candidate radioprotective drugs.

Communications medicine·2025
Same author

Assessing bioactivity of environmental water samples filtered using nanomembrane technology and mammalian cell lines.

Eco-Environment & Health·2024
Same author

Effects of microplastic interaction with persistent organic pollutants on the activity of the aryl hydrocarbon and estrogen receptors.

Chemosphere·2024
Same author

The Role of Extracellular Vesicles in the Skin and Their Interactions with Nanoparticles.

World journal of nanoscience and nanotechnology·2024

Area of Science:

  • Biosensor technology
  • Surface chemistry
  • Biomolecular engineering

Background:

  • Biosensor design requires optimizing receptor immobilization and target detection.
  • Steric crowding is a known challenge in surface-based assays.
  • Porous silicon (PSi) offers a versatile platform for biosensor fabrication.

Purpose of the Study:

  • To quantify steric crowding effects on antibody receptor immobilization and target detection.
  • To optimize the biotin/streptavidin immobilization system for biosensor applications.
  • To investigate the influence of substrate topography on binding interactions.

Main Methods:

  • Fabrication of a label-free optical biosensor using n-type macroporous silicon (PSi).
  • Implementation of a sandwich assay with biotin/streptavidin linking chemistry.

Related Experiment Videos

  • Bottom-up analysis of each layer (biotin, streptavidin, receptor) to assess steric crowding.

    • Main Results:

    • Steric crowding was observed to impede binding at each layer of the sandwich assay.
    • Onset of steric crowding in biotin layer occurred at 57% surface coverage on heterogeneous PSi.
    • Preblocking streptavidin binding sites reduced steric crowding, increasing receptor immobilization.

    Conclusions:

    • Surface topography significantly influences steric crowding compared to planar surfaces.
    • Optimizing linker chemistry and considering binding avidity are crucial for effective biosensor design.
    • This study provides insights for enhancing the performance of chip-based biosensors.