Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electroosmosis-based nanopipettor.

Chang Kyu Byun1, Xiayan Wang, Qiaosheng Pu

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79407, USA.

Analytical Chemistry
|April 13, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ferroptosis Revealed by a Bifunctional Nanopipette Sensing the Intracellular Iron-Reactive Oxygen under Dibenzothiophene Exposure.

Analytical chemistry·2026
Same author

Concentration Gradient-Controlled Electrochemical Etching: A Universal Strategy for Nanoelectrodes with Tunable Geometries.

Small methods·2026
Same author

MicroRNAs in Obesity-Associated Metabolic Inflammation: From Molecular Mechanisms to Clinical Translation.

Diabetes/metabolism research and reviews·2026
Same author

Dual-Site Synergy in PtRuFeCoNi High-Entropy Alloys: Mitigating OH*/H* Adsorption Competition for Efficient Alkaline Hydrogen Evolution.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Chip-adaptable multichannel resistance meter enables integrity monitoring for an arrayed biological barrier chip.

Biosensors & bioelectronics·2026
Same author

Assessment of Micromixer Design in Microfluidic Chips via Mixing Efficiency Measurement Using Contactless Conductivity Detection.

Analytical chemistry·2026
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Researchers developed a novel electroosmotic nanopipettor for precise picoliter liquid handling. This moving-part-free device enables accurate and reproducible transfer of nanoliter-to-picoliter volumes, crucial for single-cell analysis and reducing assay costs.

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Microfluidics and Nanotechnology

Background:

  • High-throughput analysis requires reduced reagent consumption for cost-effectiveness.
  • Investigating individual cell behavior necessitates precise picoliter liquid handling.
  • Existing pipetting technologies struggle with accuracy and precision below 100 nL.

Purpose of the Study:

  • To develop a novel nanopipettor capable of handling nanoliter-to-picoliter volumes.
  • To address the limitations of current pipetting technologies for single-cell analysis.
  • To create a cost-effective solution for high-throughput laboratories by reducing reagent usage.

Main Methods:

  • Development of an electroosmotic (EO) flow pump integrated with a grounding interface and a specialized pipet tip.
  • Microfabrication techniques were employed for the EO pump construction.

Related Experiment Videos

  • Characterization of the pipettor's performance in terms of accuracy and reproducibility across various volumes.

    • Main Results:

    • The developed EO-driven nanopipettor demonstrated excellent reproducibility (RSD = 6% at 140 pL, 2% at 950 pL, 2% at 13 nL).
    • High accuracy was achieved, with errors of 9% at 0.13 nL, 4% at 1.0 nL, and 3% at 10 nL.
    • The pipettor successfully aliquoted and transported nanoliter-to-picoliter volumes without moving parts.

    Conclusions:

    • The electroosmosis-based nanopipettor offers a viable solution for precise picoliter liquid handling.
    • The device's moving-part-free design enhances reliability and simplifies operation.
    • This technology has the potential to significantly reduce costs and improve efficiency in high-throughput single-cell analysis.