Chip-adaptable multichannel resistance meter enables integrity monitoring for an arrayed biological barrier chip

Transendothelial/epithelial electrical resistance (TEER) measurement is a gold standard for quantitative evaluation of barrier integrity, which is crucial for barrier function characterization. Recent strategies to realize TEER monitoring for biological barrier chips require specific designs on chips for the introduction of the measurement apparatus; the lack of a device with high adaptability to various types of barrier chips is still a bottleneck in this field. Herein, we fabricated a chip-adaptable multichannel resistance meter (CAMRM) that enables real-time recording of a four-channel TEER signal simultaneously. Through adapting to the arrayed barrier chip, TEER dynamics during barrier formation were monitored, as indicated by a gradual increase followed by a plateau. Furthermore, CAMRM allowed simultaneous recording of the TEER for both healthy and inflammatory barriers reconstructed on an arrayed chip, identifying progressively decreased barrier integrity under inflammation induction. This work provides a powerful and reliable tool for real-time and parallel integrity monitoring across various multiplexed biological barrier chips, which exhibits remarkable potential for advancing high-throughput disease-associated mechanism study and drug screening.