Related Experiment Video
Updated: Jul 15, 2026
An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
[Determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection by HPLC]
Juan Tian1, Wei-hao Wang, Hui-min Gao
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Objective:
To establish a high performance liquid chromatography (HPLC) method for determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection.
Method:
Alltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol (80:10:10) as the mobile phase and the detection wavelength was at 220 nm.
Result:
The mean recovery of matrine, sophoridine and oxymatrine in Compound Kushen Injection was 99.51% (RSD 1. 58%), 99.24% (RSD 1.44%) and 100.22% (RSD 1.85%), respectively.
Conclusion:
The method was simple, rapid, accurate and specific and suitable for quality control of the product.
More Related Videos
10:38Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
Published on: May 9, 2020
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019