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Related Experiment Video

Updated: Feb 17, 2026

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
07:44

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Published on: October 20, 2018

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[Cell sheet engineering].

Teruo Okano1

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.

Rinsho Shinkeigaku = Clinical Neurology
|April 17, 2007
PubMed
Summary
This summary is machine-generated.

Cell sheet engineering utilizes temperature-responsive surfaces for easy cell sheet harvesting. This innovative technique advances tissue engineering and regenerative medicine, enabling new therapies and complex tissue fabrication.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Context:

  • Cell sheets are cultivated on temperature-responsive surfaces (poly N-isopropylacrylamide) at 37°C.
  • Harvesting and manipulation are achieved via temperature shifts to 20°C, exploiting surface property changes.

Purpose:

  • To introduce cell sheet engineering as a novel platform for tissue regeneration.
  • To demonstrate the fabrication of various human tissues and in vitro organoid structures.

Summary:

  • Temperature-responsive surfaces facilitate the non-invasive preparation and handling of cell sheets.
  • Diverse cell types, including epithelial, endothelial, and cardiac cells, have been successfully cultured and harvested.
  • Layered cell sheets enable the creation of complex, functional tissue constructs, including beating heart tissue and organoid models.

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Micro-scale Engineering for Cell Biology
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Related Experiment Videos

Last Updated: Feb 17, 2026

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Published on: October 20, 2018

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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
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Micro-scale Engineering for Cell Biology
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Impact:

  • Cell sheet engineering provides a promising avenue for novel therapies in regenerative medicine.
  • Clinical applications are emerging, particularly for corneal surface diseases.
  • This technology facilitates the development of in vitro models for complex organs like the liver and kidney.