Wavelength shifts in solid-state circular dichroism spectra: a possible explanation
Related Experiment Video
Updated: Jul 15, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Ettore Castiglioni1, Sergio Abbate, Giovanna Longhi
1JASCO Corporation, Hachioji-shi, Tokyo, Japan. ettore.castiglioni@jasco-europe.com
Abstract:
We have devised an artificial sampling approach generating "absorption flattening" (AF) on the UV-Vis spectrum of a solution of a chiral compound: Tris (ethylendiamine)cobalt(III) chloride (Lambda-Co(en)(3)Cl(3)). We have observed a concomitant red shift of the CD maxima. Related CD and absorption spectra have been calculated from spectra recorded on diluted homogeneous solutions, thus monitoring the effect of AF on both types of data. Experimental data are in good agreement with calculated spectra. Simulations with suitable bandshapes show that the red shift of the CD spectrum is due to AF. On the basis of these results, we conclude that AF is an important cause of distortions in CD spectra for inhomogeneous samples. Plans to compensate or at least to take into account this effect are presented.
Related Concept Videos
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
The de Broglie Wavelength
NMR Spectroscopy: Chemical Shift Overview
For instance, the proton...
π Electron Effects on Chemical Shift: Aromatic and Antiaromatic Compounds
UV–Vis Spectroscopy: Molecular Electronic Transitions
π Electron Effects on Chemical Shift: Overview

