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Published on: May 10, 2024
[Relationship between 5,10-methylenetetrahydrofolate reductase genetic polymorphism and arsenic metabolism]
Fu-rong Deng1, Xin-biao Guo, Li Chen
1Department of Occupational & Enviromental Health Sciences, Peking University School of Public Health, Beijing 100083,China.
Objective:
To study the relationship between 5,10-methylenetetrahydrofolate reductase(MTHFR) genetic polymorphism and arsenic metabolism and the role of folate in it.
Methods:
Forty-three individuals who were exposed to arsenic more than 0.05 mg/L in well water were chosen and the vein blood and morning urine was collected; The MTHFR C677 polymorphism was analyzed by PCR-RFLP method ; Urinary inorganic and methylated arsenic were speciated by high performance liquid chromatography combined with hydride-generation atomic fluorescence spectrometry; Microbiological assay was used to estimate serum folate.
Results:
Subjects with the genotype of CT/TT had the increased percentage of urinary As3+ (r=27.17, P=0.029) and decreased percentage of urinary dimethylarsinic acid (r=26.57, P=0.033). No association was observed among serum folate, genetic polymorphisms of MTHFR C677 and the percentage of urinary arsenic (P>0.05).
Conclusion:
Genetic polymorphism of MTHFR C677 was significantly associated with arsenic methylation and this effect was not associated with the serum folate.
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