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Published on: July 17, 2021
[Genetic polymorphisms at five STR loci in Chengdu Han population]
Xiao Chen1, Yan-Hua Song, Ying Li
1Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Summary
This study analyzed genetic variations at five short tandem repeat (STR) loci in the Chengdu Han population. The findings provide valuable data for forensic applications and population genetics research.
Area of Science:
- Forensic Genetics
- Population Genetics
- Human Genetics
Background:
- Short tandem repeat (STR) loci are crucial for human identification.
- Understanding population-specific genetic diversity is essential for forensic science.
Purpose of the Study:
- To characterize allele structure and genetic polymorphisms at five STR loci in the Chengdu Han population.
- To establish a preliminary genetic database for this population.
- To evaluate the forensic value of these STR loci.
Main Methods:
- DNA extraction from blood samples using the Chelex-100 method.
- Polymerase Chain Reaction (PCR) amplification of five STR loci.
- Polyacrylamide Gel Electrophoresis (PAGE) and gel silver staining for analysis.
Main Results:
- Allele counts ranged from 5 to 10 across the five STR loci.
- Observed heterozygosity varied from 64% to 83%.
- Discrimination powers ranged from 84.3% to 95.44%, indicating high discriminatory potential.
Conclusions:
- The analyzed STR loci exhibit high discrimination power, suitable for forensic identification.
- The generated data contribute to forensic databases and population genetics studies.
- All loci were found to be in Hardy-Weinberg equilibrium, supporting their reliability.
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