Related Experiment Video
Updated: Jul 15, 2026
Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
Published on: July 27, 2016
[Purification of human VLDL apolipoproteins by middle pressure liquid chromatography]
Yan-Fei Xin1, Yun Duan, Zu-Yue Deng
1Apolipoprotein Research Unit, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To purify human VLDL apolipoproteins by middle-pressure liquid chromatography.
Methods:
Human VLDLs were isolated by one step density ultracentrifugation. Delipided human VLDL was separated by Sephacryl S-200 molecular sieve chromatography. ApoE was purified by heparin Sepharose CL-6B affinity chromatography. ApoC I ,C II and C III were purified from apoC. fraction by DEAE-Sephacel ion exchange chromatography:
Results:
Purified apoE, apoC I, apoC II and apoC III were obtained. SDS-PAGE and immunodiffusion tests indicated the isolated proteins were pure.
Conclusion:
We have established a purification procedure for human VLDL apolipoproteins with highly efficiency and simplicity by MPLC.
