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Column liquid chromatographic determination of carbadox and olaquindox in feeds
F J dos Ramos1, I N da Silveira, G de Graaf
1Laboratório de Bromatologia e Nutrição, Faculdade de Farmácia da Universidade de Coimbra, Portugal.
Abstract:
A column liquid chromatographic method for simultaneous determination of carbadox and olaquindox in swine feeds is described. The drugs were extracted from feeds with carbon tetrachloride-dimethylformamide (80:20) at 60 degrees C for 30 min. The extract was mixed with water (25:45). After centrifugation the aqueous layer was chromatographed on a reversed-phase column using gradient elution and ultraviolet detection at wavelengths of 305 and 262 nm. Recoveries from samples fortified at levels of 20-50 ppm were 92 +/- 9% for carbadox and 93 +/- 6% for olaquindox (means +/- standard deviations, n = 71).