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Fast determination of conditions for maximum dynamic capacity in cation-exchange chromatography of human monoclonal

Alexander Faude1, Dörthe Zacher, Egbert Müller

  • 1Institute of Cell Biology and Immunology, University of Stuttgart, Stuttgart, Germany. alexander.faude@izi.uni-stuttgart.de

Journal of Chromatography. A
|April 20, 2007
PubMed

Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to the Hofmeister series. As this downshift correlates well with zeta potential values, a measurement of the latter allows the determination of the pH value for maximum DBC under a given set of conditions. Thus, the use of zeta potential values can accelerate the purification process development and helps to understand the protein adsorption mechanism.

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