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Updated: Jul 15, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
Published on: June 30, 2022
Coevolution of genomic intron number and splice sites
Manuel Irimia1, David Penny, Scott W Roy
1Allan Wilson Centre for Molecular Evolution and Ecology, Massey University, Palmerston North, New Zealand.
Abstract:
Spliceosomal intron numbers and boundary sequences vary dramatically in eukaryotes. We found a striking correspondence between low intron number and strong sequence conservation of 5' splice sites (5'ss) across eukaryotic genomes. The phylogenetic pattern suggests that ancestral 5'ss were relatively weakly conserved, but that some lineages independently underwent both major intron loss and 5'ss strengthening. It seems that eukaryotic ancestors had relatively large intron numbers and 'weak' 5'ss, a pattern associated with frequent alternative splicing in modern organisms.
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