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Assay of mitochondrial ATP synthesis in animal cells and tissues

Cristofol Vives-Bauza1, Lichuan Yang, Giovanni Manfredi

  • 1Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, NY 10021, USA.

Methods in Cell Biology
|April 21, 2007
PubMed
Abstract

No abstract available in PubMed .

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In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased ATP...