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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Published on: August 5, 2009
Multiple rotation assessment through isothetic fringes in speckle photography
Luciano Angel1, Myrian Tebaldi, Néstor Bolognini
1Grupo de Optica Aplicada, Departamento de Ciencias Básicas, Universidad EAFIT, Colombia.
Abstract:
The use of different pupils for storing each speckled image in speckle photography is employed to determine multiple in-plane rotations. The method consists of recording a four-exposure specklegram where the rotations are done between exposures. This specklegram is then optically processed in a whole field approach rendering isothetic fringes, which give detailed information about the multiple rotations. It is experimentally demonstrated that the proposed arrangement permits the depiction of six isothetics in order to measure either six different angles or three nonparallel components for two local general in-plane displacements.
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