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On-chip surface-based detection with nanohole arrays.

Angela De Leebeeck1, L K Swaroop Kumar, Victoria de Lange

  • 1Department of Mechanical Engineering, University of Victoria, Victoria, BC, Canada.

Analytical Chemistry
|April 24, 2007
PubMed
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This study demonstrates a novel microfluidic device using nanohole arrays for surface plasmon resonance (SPR) sensing. This miniaturized sensor offers sensitive detection of chemical and biological analytes on-chip.

Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Surface Plasmon Resonance (SPR) is a powerful surface analysis technique.
  • Integrating SPR into microfluidic devices faces challenges with optics and surface area.
  • Nanohole arrays offer a miniaturized SPR sensing solution leveraging light transmission.

Purpose of the Study:

  • To demonstrate a microfluidic device with integrated SPR sensors using nanohole arrays.
  • To overcome limitations of traditional SPR in microfluidic platforms.
  • To develop a miniaturized, surface-sensitive diagnostic tool for microfluidics.

Main Methods:

  • Fabrication of a microfluidic device with gold film nanohole arrays.
  • Utilizing resonant light transmission through subwavelength apertures for SPR.

Related Experiment Videos

  • On-chip proof-of-concept measurements for refractive index changes and biochemical assays.

    • Main Results:

    • Demonstrated a sensitivity of 333 nm/RIU (refractive index unit).
    • Successfully detected spatial microfluidic concentration gradients.
    • Monitored a biotin-streptavidin binding interaction on-chip.

    Conclusions:

    • Nanohole arrays are effective SPR sensing elements in microfluidic platforms.
    • The device provides miniaturized, surface-sensitive diagnostic capabilities.
    • This technology enhances analytical tools for microfluidic applications.