Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Drugs Affecting Neurotransmitter Synthesis

Drugs Affecting Neurotransmitter Synthesis

Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase, which converts...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pharmacological Actions of Potassium Channel Openers on Voltage-Gated Potassium Channels.

Pharmaceuticals (Basel, Switzerland)·2025

Same author

RNA Sequencing Identified Differentially Expressed Genes in the Mesocorticolimbic and Nigrostriatal Systems of Compulsive METH-Taking Rats.

Cells·2025

Same author

PGx-Based in silico Analyses Identifies the Interactive Role of Genes, Glucose Metabolism and Dopaminergic Dysfunctional Pathways with Chronic Cocaine use and Misuse.

Current neuropharmacology·2025

Same author

Pediatric asthma population risk stratification using k-means clustering.

The Journal of asthma : official journal of the Association for the Care of Asthma·2025

Same author

Escalated oxycodone self-administration is associated with expression of voltage gated and calcium activated potassium channels in the mesocorticolimbic system in rats.

Frontiers in pharmacology·2025

Same author

Escalated Oxycodone Self-Administration Is Associated with Activation of Specific Gene Networks in the Rat Dorsal Striatum.

International journal of molecular sciences·2025

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

Published on: November 5, 2008

Neonatal dopamine depletion induces changes in morphogenesis and gene expression in the developing cortex.

Irina N Krasnova¹, Elizabeth S Betts, Abiola Dada

¹Molecular Neuropsychiatry Branch, National Institute on Drug Abuse, National Institutes of Health/DHHS, Bethesda, MD 20892, USA.

Neurotoxicity Research

|April 24, 2007

Summary

Neonatal dopamine (DA) depletion in mice alters cortical development, leading to structural changes and impaired cognitive functions like response inhibition. This highlights DA

More Related Videos

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Published on: April 3, 2013

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Published on: September 24, 2013

Related Experiment Videos

Last Updated: Jul 15, 2026

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

Published on: November 5, 2008

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Published on: April 3, 2013

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Published on: September 24, 2013

Area of Science:

Neuroscience
Developmental Biology
Molecular Biology

Background:

The mesocorticolimbic dopamine (DA) system is crucial for cognitive functions and cortical development.
Dysregulation of DA is linked to various mental health disorders affecting attention and impulse control.

Purpose of the Study:

To investigate the morphological, behavioral, and transcriptional effects of selective DA deficit in the fronto-parietal cortex during early development.
To identify molecular targets involved in DA-mediated developmental regulation and associated cognitive impairments.

Main Methods:

Focal injections of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle of BALB/c mice on postnatal day 1 (PND1).
Assessment of DA levels, cortical morphology (histology), cognitive function (object recognition), and gene expression (microarrays) at various time points.

Main Results:

Neonatal 6-OHDA treatment reduced cortical DA levels and induced significant alterations in cortical width by PND120.
Adult mice exhibited impaired response inhibition in open field object recognition tests.
Transcriptional analysis revealed significant up-regulation and down-regulation of genes involved in cytoskeletal formation and axonal guidance.

Conclusions:

Neonatal DA depletion profoundly impacts cortical development, leading to structural and functional deficits in adulthood.
Altered gene expression related to cytoskeletal dynamics and neuronal pathway finding underlies these impairments.
Findings suggest specific molecular pathways that may be targeted for conditions involving disrupted developmental DA regulation.