Related Experiment Video
Updated: Jul 15, 2026
05:33
Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
Published on: July 5, 2024
OligoCalc: an online oligonucleotide properties calculator
1Robert H. Lurie Comprehensive Cancer Center, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. wakibbe@northwestern.edu
Nucleic Acids Research
|April 25, 2007
Summary
OligoCalc is a user-friendly web tool for calculating DNA and RNA properties, including melting temperature and molecular weight. This computational engine provides essential data for nucleic acid research.
Area of Science:
- Biochemistry
- Bioinformatics
- Computational Biology
Background:
- Accurate calculation of oligonucleotide properties is crucial for molecular biology experiments.
- Existing tools may lack comprehensive features or user-friendly interfaces.
Purpose of the Study:
- To develop a web-accessible computational engine, OligoCalc, for calculating various DNA and RNA properties.
- To provide a user-friendly interface for researchers to access critical oligonucleotide data.
Main Methods:
- OligoCalc is a client-based computational engine accessible via a web browser.
- It calculates properties such as molecular weight, concentration, melting temperature, and absorbance coefficients.
- Incorporates three methods for melting temperature calculation, including a nearest-neighbor thermodynamic model.
Main Results:
- OligoCalc provides a comprehensive suite of calculations for single-stranded and double-stranded DNA and RNA.
- The tool features an intuitive 'calculator' interface for ease of use.
- Since 1997, OligoCalc has seen over 900,000 accesses from numerous distinct hosts.
Conclusions:
- OligoCalc serves as a valuable and widely adopted resource for nucleic acid property calculations.
- Its accessibility and comprehensive features make it an indispensable tool for researchers in molecular biology and related fields.
