Related Experiment Video
Updated: Jul 15, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Can methicillin-resistant Staphylococcus aureus be found in an ambulance fleet?
Chad E Roline1, Christina Crumpecker, Thomas M Dunn
1Georgetown University School of Medicine, Washington, DC 20007, USA. cer28@georgetown.edu
Methicillin-resistant Staphylococcus aureus (MRSA) was found in nearly half of ambulances surveyed, indicating a potential contamination risk. This highlights the need for enhanced cleaning protocols in emergency medical services (EMS) vehicles.
Area of Science:
- Infectious Disease Epidemiology
- Microbiology
- Public Health
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) is a significant public health concern.
- Healthcare environments are known reservoirs for MRSA transmission.
- The potential for MRSA contamination in emergency medical services (EMS) vehicles is not well-established.
Purpose of the Study:
- To conduct an initial screening for MRSA contamination within an ambulance fleet.
- To assess the prevalence of MRSA in emergency medical services (EMS) vehicles.
Main Methods:
- A cross-sectional study was conducted in June 2006.
- Samples were collected from five distinct areas in 21 ambulances (n=105).
- Dacron swabs and mannitol salt agar with oxacillin were used for MRSA detection.
Main Results:
- MRSA contamination was detected in 10 out of 21 ambulances (47.6%).
- Thirteen positive samples were isolated from the tested fleet.
- A significant prevalence of MRSA was observed in the sampled ambulances.
Conclusions:
- Ambulances may harbor substantial MRSA contamination.
- EMS vehicles could serve as reservoirs for MRSA transmission.
- Further investigation into MRSA screening and disinfection in ambulances is warranted.
Related Concept Videos
Clinical Significance of Antibiotic Resistance
Mechanism of Antibiotic Resistance in MRSA
Staphylococcal Skin Infections
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Development of Antibiotic Resistance