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Updated: Jul 15, 2026
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
[Saponins from roots of Hedysarum multijugum]
Wei Wang1, Li-Qian Hai, Yu-Ying Zhao
1Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
Objective:
To study the chemical constituents from the root of Hedysarum multijugum.
Method:
The compounds were separated by chromatographic methods, their structures were identified by spectral analysis.
Result:
Four compounds were isolated and identified as soyasaponin II methyl ester (1), soyasaponin II (2), soyasaponin Bg (3), and soyasaponin I (4).
Conclusion:
The four compounds were obtained from the plant for the first time. Compound 3 was isolated from the genus Hedysarum for the first time, and the NMR data of 3 was reported for the first time.
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