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Mitochondrial DNA alterations in aging.

Kamile Oztürk¹, Abdullah Olgun, Ercan Saruhan

¹Department of Biochemistry and Clinical Biochemistry, Gülhane School of Medicine, 06018 Etlik, Ankara, Turkey. kamileztrk@yahoo.com.tr

Annals of the New York Academy of Sciences

|April 27, 2007

Summary

Mitochondrial DNA (mtDNA) deletions did not increase with aging in a 2.4-kb region. Further sequencing is needed to detect age-dependent changes in mitochondrial DNA alterations.

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Area of Science:

Genetics
Molecular Biology
Aging Research

Background:

Mitochondrial DNA (mtDNA) alterations are associated with aging.
Investigating specific mtDNA regions can reveal age-related changes.

Purpose of the Study:

To investigate deletions/insertions in a 2.4-kb region of mtDNA, including the D-loop, in relation to aging.
To determine if major mtDNA deletions increase with age.

Main Methods:

Screened 96 individuals aged 20-94 years.
Purified genomic DNA from whole blood.
Amplified the 2.4-kb mtDNA region using PCR and visualized via agarose gel electrophoresis.
Sequenced one amplicon for confirmation.

Main Results:

Detected mtDNA deletions in only two younger individuals (ages 26 and 30) at this resolution.
No significant increase in major deletions/insertions was observed in the analyzed mtDNA region with aging.

Conclusions:

The analyzed 2.4-kb mtDNA region does not show an age-dependent increase in major deletions/insertions.
Complete sequencing of this mtDNA region is necessary to identify potential age-related changes.