2,5-Hexanedione exposure alters the rat Sertoli cell cytoskeleton. II. Intermediate filaments and actin

The effects of 2,5-hexanedione (2,5-HD) exposure on the expression of intermediate filament and actin proteins in adult rat testis was examined during progressive stages of testicular injury. The distribution of vimentin within testis cross sections was examined using immunohistochemistry while rhodamine-labeled phalloidin was used to localize filamentous actin. Keratin was not detected in immunoblots of intermediate filament proteins tested with an antikeratin antibody to investigate the possible reexpression of the prepubertal intermediate filament protein keratin in 2,5-HD-exposed adult testes. However, 2,5-HD exposure did result in a progressively abnormal distribution of actin and vimentin within the seminiferous epithelium with the appearance of a high-molecular-weight protein which was vimentin immunoreactive and not present in control testes.