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Related Experiment Video

Updated: Jul 15, 2026

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Published on: September 30, 2014

Uberolysin: a novel cyclic bacteriocin produced by Streptococcus uberis.

Ruth E Wirawan¹, Kara M Swanson¹, Torsten Kleffmann²

¹Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, PO Box 56, Dunedin, New Zealand.

Microbiology (Reading, England)

|April 28, 2007

Summary

Streptococcus uberis, a cause of bovine mastitis, produces a novel cyclic bacteriocin called uberolysin. This antimicrobial peptide lyses susceptible bacteria and its gene is found in many S. uberis strains.

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Published on: July 25, 2017

Area of Science:

Microbiology
Bacteriocins
Bovine Mastitis

Background:

Streptococcus uberis is an environmental bacterium and a primary cause of bovine mastitis.
S. uberis produces bacteriocin-like inhibitory substances, including nisin U.
Previous research identified nisin U from S. uberis strain 42.

Purpose of the Study:

To characterize a second bacteriocin produced by S. uberis strain 42.
To investigate the genetic basis and properties of this novel bacteriocin, named uberolysin.

Main Methods:

Isolation and biochemical characterization of uberolysin.
Transposon mutagenesis to generate a mutant and identify the gene cluster.
DNA sequencing to analyze the gene cluster and flanking regions.
Enzymic hydrolysis to confirm precursor and processing.

Main Results:

Uberolysin is a 7048 Da peptide that lyses target bacteria.
The structural gene (ublA) and associated genes for modification, transport, and immunity were identified.
Uberolysin is a unique cyclic bacteriocin with a short leader sequence, modified post-translationally.
The ublA gene was found in 18% of tested S. uberis strains.

Conclusions:

Uberolysin is a novel cyclic bacteriocin produced by S. uberis.
Its genetic cluster provides insights into bacteriocin biosynthesis and regulation.
Uberolysin represents a unique class of antimicrobial agents with potential applications.