Related Experiment Video
Updated: Jul 15, 2026

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Single versus double-gloving for obstetric and gynecologic procedures.
Christie Lancaster1, Patrick Duff
1Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville, FL, USA.
Double-gloving significantly reduces the risk of blood-skin exposure during pelvic surgery compared to single-gloving. While overall perforation rates were similar, double-gloving offers superior protection against hazardous exposures.
Area of Science:
- Surgical Safety
- Infection Control
- Medical Device Performance
Background:
- Surgical glove perforation is a persistent concern in operating rooms.
- Understanding perforation rates is crucial for preventing surgical site infections and healthcare worker exposure.
Purpose of the Study:
- To compare the incidence of glove perforations between single-glove and double-glove techniques.
- To assess the risk of blood-skin exposure associated with each gloving method.
Main Methods:
- A prospective cohort study involving 1000 glove sets (675 double, 325 single) from pelvic surgery procedures.
- Gloves were collected post-procedure and systematically analyzed for perforations.
- Data stratified by procedure type and surgical team member.
Main Results:
- No significant difference in overall glove perforation rates between single (11%) and double-gloving (10%).
- A significantly higher rate of corresponding defects in both gloves of a double-glove set (2%) versus single-glove perforations (11%) was observed.
- Major gynecologic procedures exhibited the highest perforation rate (19%), with surgical nurses most frequently sustaining glove injuries.
Conclusions:
- Double-gloving is recommended for all pelvic surgery procedures to minimize blood-skin exposure risks.
- While overall perforation rates may not differ, double-gloving provides a critical safety barrier against direct blood exposure.
Related Concept Videos
PPE Use in Healthcare Settings II: Doffing
PPE Use in Healthcare Settings I: Donning
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
Handwashing II: Pre-procedure and Initial Procedure Steps
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail paint...
Hand hygiene
Hand washing...
Handwashing III: During the Procedure and Post-Procedure Steps
