Related Experiment Video
Updated: Jul 15, 2026

Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons
Published on: August 16, 2020
Parkin and synphilin-1 isoform expression changes in Lewy body diseases
Jordi Humbert1, Katrin Beyer, Cristina Carrato
1Departments of Pathology, Hospital Germans Trias i Pujol, Autonomous University of Barcelona, 08916 Badalona, Spain.
Abstract:
Alternative splicing gives rise to at least seven parkin and eight synphilin-1 isoforms. Since both parkin and synphilin-1 have been involved in Lewy body (LB) formation, we decided to explore whether their isoforms are differentially expressed in LB diseases. With this aim, we studied relative mRNA expression levels of parkin and synphilin-1 isoforms in the frontal cortices of patients with dementia with LBs, the LB variant of Alzheimer's disease and Parkinson's disease and compared the findings with those obtained from Alzheimer's disease patients and control individuals. Duplex real-time PCR reactions, with beta-actin as internal standard, were carried out in a LightCycler. mRNA expression levels of parkin and synphilin-1 isoforms were seen to be specifically altered in each of the LB diseases studied. These findings suggest that parkin and synphilin-1 isoform expression changes play a significant role in the pathogenesis of LB diseases.
Related Concept Videos
Parkinson Disease ll: Pathophysiology
Neural Regulation
Parkinson Disease l: Introduction
Lysosomal Hydrolases
Parkinson's Disease: Overview
Alterations in Muscle Tone lll

