Related Experiment Video
Updated: Jul 15, 2026

Induction and Testing of Hypoxia in Cell Culture
Published on: August 12, 2011
Triphlorethol-A induces heme oxygenase-1 via activation of ERK and NF-E2 related factor 2 transcription factor
Kyoung Ah Kang1, Kyoung Hwa Lee, Jae Woo Park
1Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju 690-756, Republic of Korea.
Abstract:
Triphlorethol-A, phlorotannin found in Ecklonia cava, induced heme oxygenase-1 (HO-1) expression at mRNA and protein levels, leading to increased HO-1 activity. Transcription factor NF-E2 related factor 2 (Nrf2) regulates antioxidant response element (ARE) of phase 2 detoxifying and antioxidant enzymes. Triphlorethol-A increased nuclear translocation, ARE binding, and transcriptional activity of Nrf2. Triphlorethol-A exhibited activation of ERK and U0126, inhibitor of ERK kinase, suppressed triphlorethol-A induced activation of Nrf2, finally decreased HO-1 protein level. Taken together, these data suggest that triphlorethol-A augments cellular antioxidant defense capacity through induction of HO-1 via ERK-Nrf2-ARE signaling pathway, thereby protecting cells from oxidative stress.
Related Concept Videos
Regulation of Angiogenesis and Blood Supply
Master Transcription Regulators
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Regulation of the Unfolded Protein Response
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
The Unfolded Protein Response
